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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
17/03/2011 |
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Data da última atualização: |
28/10/2011 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
SILVESTRINI, M.; RAMIRO, D. A.; GUERREIRO-FILHO, O.; MALUF, M. P. |
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Afiliação: |
Coffee Center "Alcides Carvalho"; IAC; IAC; MIRIAN PEREZ MALUF, SAPC. |
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Título: |
Defense-related gene expression in response to leaf-miner infection. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas. |
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Idioma: |
Inglês |
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Conteúdo: |
In Brazilian coffee plantations, the leaf-miner (Leucoptera coffeella) represents a major threat. Insect control is mainly based on the use of pesticides, as resistant cultivars are not yet available. However, the coffee breeding program of IAC has already selected promising genotypes bearing resistance to the leaf-miner, which was transferred from the diploid species Coffea racemosa. In order to characterize molecular aspects of defense mechanisms associated to the resistance response, expression of key genes was evaluated in coffee leaves, from both resistant and susceptible plants, in response to leaf-miner infection. Infected leavesb were collected at different time-intervals during insect development. Defense-related genes were identified in the Coffee Genome Database, through Blast searches. Gene specific primers were used to amplify corresponding transcripts on sampled leaves using a quantitative RT-PCR approach. Results indicated that there are no significant differences in the expression patterns of evaluated genes when comparing resistant and susceptible infected leaves. Major differences were observed for lipoxygenase, glutathione transferase, protein-kinase receptor and glucanase. However, these differences are mainly associated with expression timing along insect infection rather than with gene regulation, suggesting that resistance to leafminer in coffee may be associated with a previously built basal defense-response. |
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Thesagro: |
Leucoptera Coffeella. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01958nam a2200157 a 4500
001 1881314
005 2011-10-28
008 2008 bl uuuu u00u1 u #d
100 1 $aSILVESTRINI, M.
245 $aDefense-related gene expression in response to leaf-miner infection.$h[electronic resource]
260 $aIn: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas.$c2008
520 $aIn Brazilian coffee plantations, the leaf-miner (Leucoptera coffeella) represents a major threat. Insect control is mainly based on the use of pesticides, as resistant cultivars are not yet available. However, the coffee breeding program of IAC has already selected promising genotypes bearing resistance to the leaf-miner, which was transferred from the diploid species Coffea racemosa. In order to characterize molecular aspects of defense mechanisms associated to the resistance response, expression of key genes was evaluated in coffee leaves, from both resistant and susceptible plants, in response to leaf-miner infection. Infected leavesb were collected at different time-intervals during insect development. Defense-related genes were identified in the Coffee Genome Database, through Blast searches. Gene specific primers were used to amplify corresponding transcripts on sampled leaves using a quantitative RT-PCR approach. Results indicated that there are no significant differences in the expression patterns of evaluated genes when comparing resistant and susceptible infected leaves. Major differences were observed for lipoxygenase, glutathione transferase, protein-kinase receptor and glucanase. However, these differences are mainly associated with expression timing along insect infection rather than with gene regulation, suggesting that resistance to leafminer in coffee may be associated with a previously built basal defense-response.
650 $aLeucoptera Coffeella
700 1 $aRAMIRO, D. A.
700 1 $aGUERREIRO-FILHO, O.
700 1 $aMALUF, M. P.
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Registro original: |
Embrapa Café (CNPCa) |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
20/12/2018 |
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Data da última atualização: |
05/02/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
OLIVEIRA, P. S. N. de; COUTINHO, L. L.; TIZIOTO, P. C.; CESAR, A. S. M.; OLIVEIRA, G. B. de; DINIZ, W, J. da S.; LIMA, A. O. de; REECY, J. M.; MOURÃO, G. B.; ZERLOTINI NETO, A.; REGITANO, L. C. de A. |
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Afiliação: |
PRISCILA SILVA NEUBERN DE OLIVEIRA, Bolsista/Embrapa Pecuária Sudeste; LUIZ L. COUTINHO, USP; POLYANA C. TIZIOTO, NGS Genomic Solutions; ALINE S. M. CESAR, USP; GABRIELLA B. DE OLIVEIRA, USP; WELLISON J. DA S. DINIZ, UFSCar; ANDRESSA O. DE LIMA, UFSCar; JAMES M. REECY, Iowa State University; GERSON B. MOURÃO, USP; ADHEMAR ZERLOTINI NETO, CNPTIA; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
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Título: |
An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Scientific Reports, v. 8, p. 1-12, 2018. |
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DOI: |
10.1038/s41598-018-35315-5 |
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Idioma: |
Inglês |
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Notas: |
Article number: 17072. Na publicação: Adhemar Zerlotini, Luciana C. A. Regitano. |
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Conteúdo: |
Residual Feed Intake (RFI) is an economically relevant trait in beef cattle. Among the molecular regulatory mechanisms, microRNAs (miRNAs) are an important dimension in post-transcriptional regulation and have been associated with different biological pathways. Here, we performed differential miRNAs expression and weighted gene co-expression network analyses (WGCNA) to better understand the complex interactions between miRNAs and mRNAs expressed in bovine skeletal muscle and liver. MiRNA and mRNA expression data were obtained from Nelore steers that were genetically divergent for RFI (N = 10 [low RFI or feed efficient]; N = 10 [high RFI or feed inefficient]). Differentially expressed and hub miRNAs such as bta-miR-486, bta-miR-7, bta-miR15a, bta-miR-21, bta-miR 29, btamiR-30b, bta-miR-106b, bta-miR-199a-3p, bta-miR-204, and bta-miR 296 may have a potential role in variation of RFI. Functional enrichment analysis of differentially expressed (DE) miRNA?s target genes and miRNA?mRNA correlated modules revealed that insulin, lipid, immune system, oxidative stress and muscle development signaling pathways might potentially be involved in RFI in this population. Our study identified DE miRNAs, miRNA - mRNA regulatory networks and hub miRNAs related to RFI. These findings suggest a possible role of miRNAs in regulation of RFI, providing new insights into the potential molecular mechanisms that control feed efficiency in Nelore cattle. |
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Palavras-Chave: |
MicroRNAs; Residual Feed Intake. |
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Thesagro: |
Gado Nelore. |
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Thesaurus NAL: |
Beef cattle; Nellore; Skeletal muscle. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/189126/1/AP-Integrative-Adhemar-etal.pdf
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Marc: |
LEADER 02518naa a2200337 a 4500
001 2102149
005 2019-02-05
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1038/s41598-018-35315-5$2DOI
100 1 $aOLIVEIRA, P. S. N. de
245 $aAn integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle.$h[electronic resource]
260 $c2018
500 $aArticle number: 17072. Na publicação: Adhemar Zerlotini, Luciana C. A. Regitano.
520 $aResidual Feed Intake (RFI) is an economically relevant trait in beef cattle. Among the molecular regulatory mechanisms, microRNAs (miRNAs) are an important dimension in post-transcriptional regulation and have been associated with different biological pathways. Here, we performed differential miRNAs expression and weighted gene co-expression network analyses (WGCNA) to better understand the complex interactions between miRNAs and mRNAs expressed in bovine skeletal muscle and liver. MiRNA and mRNA expression data were obtained from Nelore steers that were genetically divergent for RFI (N = 10 [low RFI or feed efficient]; N = 10 [high RFI or feed inefficient]). Differentially expressed and hub miRNAs such as bta-miR-486, bta-miR-7, bta-miR15a, bta-miR-21, bta-miR 29, btamiR-30b, bta-miR-106b, bta-miR-199a-3p, bta-miR-204, and bta-miR 296 may have a potential role in variation of RFI. Functional enrichment analysis of differentially expressed (DE) miRNA?s target genes and miRNA?mRNA correlated modules revealed that insulin, lipid, immune system, oxidative stress and muscle development signaling pathways might potentially be involved in RFI in this population. Our study identified DE miRNAs, miRNA - mRNA regulatory networks and hub miRNAs related to RFI. These findings suggest a possible role of miRNAs in regulation of RFI, providing new insights into the potential molecular mechanisms that control feed efficiency in Nelore cattle.
650 $aBeef cattle
650 $aNellore
650 $aSkeletal muscle
650 $aGado Nelore
653 $aMicroRNAs
653 $aResidual Feed Intake
700 1 $aCOUTINHO, L. L.
700 1 $aTIZIOTO, P. C.
700 1 $aCESAR, A. S. M.
700 1 $aOLIVEIRA, G. B. de
700 1 $aDINIZ, W, J. da S.
700 1 $aLIMA, A. O. de
700 1 $aREECY, J. M.
700 1 $aMOURÃO, G. B.
700 1 $aZERLOTINI NETO, A.
700 1 $aREGITANO, L. C. de A.
773 $tScientific Reports$gv. 8, p. 1-12, 2018.
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